Genotypic and seasonal variations of catechin and caffeine content in exotic collection of tea [Camellia sinensis (L.) O. Kuntze] germplasm in Sri Lanka

Authors

  • J. D. Kottawa-Arachchi Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062
  • M.A.B. Ranatunga Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062
  • A.M.T. Amarakoon Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062
  • M.T.K. Gunasekare Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062
  • R.K. Sharma Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062
  • H.K. Chaudhary Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062
  • V.K. Sood Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062
  • R. Katoch Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062
  • D.K. Banyal Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062
  • E.N.U. Edirisinghe Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062
  • R.N. Attanayake Department of Genetics and Plant Breeding, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062

Keywords:

Tea quality, <i>Camellia sinensis</i>, flavan-3-ols, caffeine, seasonal variations

Abstract

The quality of made tea is largely dependent on the key metabolites viz. caffeine and flavan-3-ols. The aim of the present study was to quantify flavan-3-ols and caffeine of Sri Lankan exotic tea germplam. Fresh leaf flavan-3- ols; catechin (+C), epicatechin (EC), epicatechingallate (ECg), epigallocatechin (EGC) and epigallocatechingallate (EGCg), caffeine and gallic acid of 58 accessions (54 exotic and 4 improved cultivars) were performed by High Performance Liquid Chromatography (HPLC). Variation in metabolites among cultivars and seasons were determined. Among the flavan-3-ols, EGCg was the most abundant followed by EGC, ECg, EC and +C. Hierarchical clustering of 58 accessions based on metabolite diversity resulted in two major clusters. High caffeine containing accessions (>30 mg g-1) of Indian origin and improved TRI cultivars clustered together. Low caffeine accessions such as PBGT10, PBGT27, PBGT35, PBGT71, PBGT48, PBGT53 and PBGT54 (< 20 mg g-1) grouped separately. Higher variations in catechins and caffeine content in exotic germplasm is useful in developing tea cultivars of high quality tea.

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Published

2022-06-01

How to Cite

Kottawa-Arachchi, J. D., Ranatunga, M., Amarakoon, A., Gunasekare, M., Sharma, R., Chaudhary, H., … Attanayake, R. (2022). Genotypic and seasonal variations of catechin and caffeine content in exotic collection of tea [<i>Camellia sinensis</i> (L.) O. Kuntze] germplasm in Sri Lanka. Himachal Journal of Agricultural Research, 48(01), 8–16. Retrieved from https://hjar.org/index.php/hjar/article/view/172112

Issue

Volume 48, Issue 1, June 2022

Section

Articles

References

Balantine DA, Harbowy ME and Graham HN. 1998. Tea: the plant and its manufacture, chemistry and consumption of the beverage. In: Spiller GA (ed). Caffeine. CRC Press: Boca Raton, New York, pp. 35-72.

Banerjee B. 1992. Botanical classification of tea. In: Wilson KC and Clifford MN (eds). Tea Cultivation to Consumption. Chapman & Hall publication, London, pp. 555-601.

Cevallos-cevallos JM, Corcuera JIR, Etxeberria E, Danyluk MD and Rodrick GE. 2009. Metabolomic analysis in food science/ : a review. Trends in Food Science & Technology 20: 557-566.

Chen L and Zhou ZX. 2005. Variations of main quality components of tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in the China national germplasm tea repository. Plant Food for Human Nutrition 60: 31-35.

Chin JM, Merves ML, Goldberger BA, Sampson-Cone A and Cone EJ. 2008. Caffeine Content of Brewed Teas. Journal of Analytical Toxicology 32: 702-704.

Deka H, Barman T, Dutta J, Devi A, Tamuly P, Paul RK and Karak T. 2020. Catechin and caffeine content of tea (Camellia sinensis L.) leaf significantly differ with seasonal variation: A study on popular cultivars in North East India. Journal of Food Composition and Analysis 96:103684.

Engelhardt UH.2010. Chemistry of Tea. In: Mender L and Liu HW (eds). Comprehensive Natural Products II: Chemistry and Biology. Elsevier, United Kingdom, pp. 999-1032.

Gunasekare MTK. 2012. Tea Plant (Camellia sinensis) breeding in Sri Lanka. In: Chen L and Apostolides Z (eds). Global Tea Breeding–Achivements, Challenges and Perspectives. Springer-Verlag Berlin Heidelberg/Zhejiang press, pp. 125-176.

ISO-1573. 1980. Tea. Determination of loss in mass at 103°C. Jin JQ, Ma JQ, Ma CL, Yao MZ and Chen L. 2014.

Determination of catechin content in representative Chinese tea germplasms. Journal of Agricultural and Food Chemistry 62: 9436-9441.

Kottawa-Arachchi JD, Gunasekare MTK, Ranatunga MAB, Punyasiri PAN and Jayasinghe L. 2013. Use of biochemical compounds in tea germplasm characterization and its implications in tea breeding in Sri Lanka. Journal of the National Science Foundation of Sri Lanka 41: 309-318.

Kuribara H. 2016. Caffeine intake in the daily life: mechanism of action and safety assessment. Bulletin of Tokyo University and Graduate School of Social Welfare 6: 109-125.

Lampíø L. 2013. Varietal differentiation of white wines on the basis of phenolic compounds profile. Czech Journal of Food Sciences 31: 172-179.

Liang Z, Owens CL, Zhong G and Cheng L. 2011. Polyphenolic profiles detected in the ripe berries of Vitis vinifera germplasm. Food Chemistry 129: 940-950.

Lubanga N, Massawe F and Mayes S. 2021. Genomic and pedigree-based predictive ability for quality traits in tea (Camellia sinensis (L.) O. Kuntze). Euphytica 217: 3247.

Mohanpuria P, Kumar V, Ahuja PS and Yadav SK. 2011. Producing low-caffeine tea through post transcriptional silencing of caffeine synthase mRNA. Plant Molecular Biology 76: 3-34.

Muthiani MA, Wanyoko JK, Wachira FN, Kamunya SM, Chalo RM, Kimutai S. Khalid R and Karori S. 2016.

Potential use of Kenyan tea cultivars in development of high value diversified products potential use of Kenyan tea cultivars in development of high value diversified products. International Journal of Tea Science 12: 30-48.

Ogino A, Tanaka J, Taniguchi F, Yammamoto MP and Yamada K. 2019. A new DNA marker CafLess-TCS1 for selection of caffeine-less tea plants. Breeding Science 69: 393-400.

Ogino A, Tanaka J, Taniguchi F, Yammamoto MP and Yamada K. 2009. Detection and characterization of caffeine less tea plants originated from interspecific hybridization. Breeding Science 59: 277-283.

Price EJ, Darpal M, Perez-Fons L, Amah D, Bhattacharjee R, Heider B, Rouard M, Swennen R, Lopez-Lavalle LAB and Fraser PD. 2019. Metabolite database for root , tuber, and banana crops to facilitate modern breeding in understudied crops. The Plant Journal 101: 1258-1268.

Punyasiri PAN, Jaganathan B, Kottawa-Arachchi JD, Ranatunga MAB, Abeysinghe ISB, Gunasekare MTK and Bandara BMR. 2015. New sample preparation method for quantification of phenolic compounds of Tea (Camellia sinensis (L.) O.Kuntze): A polyphenol rich plant. Journal of Analytical Methods in Chemistry 2015.

Article ID:964341 Punyasiri PAN, Jaganathan B, Kottawa-Arachchi JD, Ranatunga MAB, Abeysinghe ISB, Gunasekare MTK and Bandara BMR. 2017. Genotypic variation in biochemical compounds of the Sri Lankan tea (Camellia sinensis L .) accessions and their relationships to quality and biotic stresses. The Journal of Horticultural Science and Biotechnology 92: 502-512.

Raina SN, Ahuja PS, Sharma RK, Das SC, Bhardwaj P, Negi R and Sharma V. 2012. Genetic structure and diversity of India hybrid tea. Genetic Resources and Crop Evolution 59: 1527-1541.

Ranatunga MAB, Kottawa-Arachchi JD, Gunasekere MTK and Yakandawala DMD. 2017. Floral diversity and genetic structure of tea germplasm of Sri Lanka. International Journal of Biodiversity: Article ID: 2957297

Robertson A. 1992. The chemistry and biochemistry of black tea production - the non-volatiles. In: Willson KC and Clifford MN (eds). Tea: Cultivation to Consumption. Chapman & Hall publication: London, pp. 555-601.

Sabhapondit S, Karak T, Bhuyan LP, Goswami BC and Hazarika M. 2012. Diversity of catechins in Notheast Indian tea cultivars. The Scientific World Journal 2012: 1-8.

Sayama K. 2015. Preventive effects of obesity by green tea and its components. Scientific Evidences for the Health Benefits of Green Tea. Japan Tea Central Public interest Incorporated Association, pp. 68-76.

Wu C, Xu H, Héritier J and Andlauer W. 2012. Determination of catechins and flavonol glycosides in Chinese tea varieties. Food Chemistry 132: 144-149.

Yao L and Xu Y. 2005. Seasonal variations of phenolic compounds in Australia-grown tea (Camellia sinensis). Journal of Agricultural and Food Chemistry 53: 6477-6483.

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