Investigating Vegetative Compatibility and Fusion Reactions among different isolates of Fusarium spp
Keywords:
Vegetative compatibility, <i>Fusarium</i>, Hyphal fusion, dual culture methodAbstract
The study was conducted to investigate the vegetative compatibility between different isolate of Fusarium to enable appropriate identification and characterization of individual isolates during July, 2024 to September, 2024, in the Department of Plant Pathology, CSKHPKV, Palampur. Microscopic examination identified four isolates as Fusarium oxysporum. Vegetative compatibility testing among the four isolates of Fusarium oxysporum using dual culture plate method and hyphal fusion assays revealed that F3 and F1 were compatible, forming heterokaryons without zone of inhibition. In contrast, other combinations exhibited zones of inhibition or coiling of hyphae, indicating incompatibility. These findings suggest that F3 and F1 belong to the same vegetative compatibility group (VCG), while others belong to different VCGs. This study provides insights into genetic relationships among Fusarium isolates and demonstrates the reliability of vegetative compatibility grouping for identifying physiological races.
Downloads
Downloads
Published
How to Cite
Issue
Section
References
Burgess T, Bihon W, Wingfield MJ andWingfield BD. 2009. A simple and rapid methodto determine vegetative compatibility groups infungi. Inoculum: Newsletter of the Mycological Society of America 60: 1-2.
Ishikawa1 FH, Souza EA, Shoji J, Connolly L, Freitag M, Read ND and Roca MG3. 2012. Heterokaryon incompatibility is suppressed following conidial anastomosis tube fusion in a fungal plant pathogen. Plos one 7(2): e31175.
Joaquim TR and Rowe RC. 1991. Vegetative compatibility and virulence of strains of Verticillium dahlia from soil and potato plants. Phytopathology 81: 552-558.
Katan T and Katan J. 1988. Vegetative-compatibility groupingsof Fusarium oxysporum f.sp.vasinfectum from tissue and the rhizosphere of cotton plants. Phytopathology 78: 852–855.
Kavita Kushwaha, Joginder Pal, DK Banyal. 2024. Endophytic and Pathogenic Fungal Root Communities Associated with Pea in Sub-humid and Dry Temperate Regions of Himachal Pradesh. Himachal Journal of Agricultural Research 50(1): 88-95.
Kumar N, Thakur BR and Singh A. 2016. Occurrence of pea root rot/wilt complex disease in Himachal Pradesh. Himachal Journal of Agricultural Research 42(1):93-98.
Leslie FJ. 1993. Fungal vegetative compatibility. Annu Review of Phytopathology 31:127–150.
Merzoug A andBelabid L. 2017. Relationship between pathogenicity, race and vegetative compatibility grouping among Algerian populations of Fusarium oxysporum f. sp. pisi causing pea wilt. Journal of Plant Protection Research 57(4).
Siddiqui IA and Shaukat SS. 2003. Combination of Pseudomonas aeruginosa and Pochonia chlamydosporia for control of root-infecting fungi in tomato. Journal of Phytopathology151: 215-222.
Sood V, Singh A, Sood S, Sood VK, Banyal DK, Sharma S and Sood T. 2024. Morpho-cultural variability of Fusarium solani isolates causing root rot of okra in low and mid hills of Himachal Pradesh. Himachal Journal of Agricultural Research 50(1):115-123.
Membership form
Guidelines to the Contributors