Association of Virus Complex with Capsicum under Protected Cultivation in Himachal Pradesh and Implications in their Management
Keywords:
Pepper Mild Mottle Virus, Capsicum, Prevalence, Detection.Abstract
Extensive surveys of capsicum crop grown under polyhouse conditions in Kangra, Mandi, Kullu, Hamirpur and Bilaspur districts of Himachal Pradesh (HP) were carried out during 2011 to 2014. The symptoms on the diseased plants included mosaic, mottling, chlorosis, upward or downward curling and deformation of leaves and fruits along with stunting of plants. The disease samples indexed through DAS ELISA showed the presence of eight viruses namely Cucumber mosaic virus (CMV); Pepper mild mottle virus (PMMoV); Tobacco mosaic virus (TMV); Tomato spotted wilt virus (TSWV); Tomato yellow leaf curl virus (TYLCV); Potato virus Y (PVY); Pepper mottle virus (PepMoV); Pepper veinal mottle virus (PVMV) in 2013 while only four viruses viz. CMV, TSWV, PVY and PMMoV were found in 2014. The presence of viruses which were found to be prevalent in DAS-ELISA was confirmed with RT-PCR using coat protein (CP) specific primers. The highest incidence of 60 per cent was recorded in Bilaspur district. The presence of three commonly occurring viruses viz. CMV, TSWV and PMMoV was confirmed with RT-PCR which yielded the amplification product of size 162 bp, 575 bp and 730 bp, respectively.Downloads
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