Morpho-cultural Variability of Fusarium Solani Isolates Causing Root Rot of Okra in Low and Mid Hills of Himachal Pradesh
Keywords:
Root rot, morpho-cultural, variability, <i>Fusarium solani<//i>.Abstract
Morphological and cultural variability was studied among the okra root rot pathogen isolates (35) of Fusarium solani collected from infested okra fields constituting three agroclimatic zones of Himachal Pradesh. The isolates cultured on potato dextrose agar medium showed considerable cultural variabilities in conidia dimensions, colony colour, colony diameter, and number of septa. Colony diameter among F. solani isolates ranged from 74-86 mm and produced white/ greyish white growth with light pink/purplish/brownish pigmentation. Aggressiveness of all the 35 isolates was tested by soil infestation method where isolates were categorized as highly, moderately, or weakly pathogenic. The isolates produced macroconidia of size ranged from 18.23-30.85×4.70-5.99 μm with 1-5 septation, microconidia of size 5.91-11.71×2.78-3.97 μm with 0-1 septation and chlamydospore of size 8-12×8-10 μm. Based on these morpho-cultural characteristics and cluster analysis using SPSS 16.0 software, all 35 isolates grown on PDA medium at 25±1 ºC were categorized into seven morpho-cultural groups and were designated as FS-MV-1 to FS-MV-7.Downloads
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